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    • Isolation and functional characterization of yeast vacuole snare complexes.

    • Isolation and functional characterization of yeast vacuole snare complexes.
    • Membrane proteins -- Physiological transport. ; Plant proteins -- Physiological transport. ; Biological transport -- Regulation. ; Plant vacuoles. Saccharomyces cerevisiae -- Physiology.
    • Eukaryotic cells are compartmentalized into membrane-enclosed organelles. Genetic screens and reconstituted biochemical assays have revealed that intracellular protein trafficking is mediated by a conserved mechanism of vesicle budding, transport,...
    • Molecular mechanisms of vacuole membrane fusion.

    • Molecular mechanisms of vacuole membrane fusion.
    • Membrane fusion. ; Tonoplasts. ; Plant vacuoles. ; Saccharomyces cerevisiae -- Molecular aspects.
    • The docking and fusion of cellular membranes plays an essential role in the secretory and endocytic pathways and in organelle inheritance. The homotypic fusion of vacuoles from the yeast Saccharomyces cerevisiae has been used as a model system to...
    • Investigation of Yip1p function in transport between the ER and the Golgi complex.

    • Investigation of Yip1p function in transport between the ER and the Golgi complex.
    • Membrane proteins -- Physiological transport. Carrier proteins. Biological transport -- Regulation. Saccharomyces cerevisiae -- Physiology. Saccharomyces cerevisiae -- Genetics. Endoplasmic reticulum. Golgi apparatus.
    • The eukaryotic secretory pathway delivers proteins and lipids to their correct destination in a regulated manner. Transport through the secretory pathway is mediated by vesicles or tubular elements that bud from donor compartments. These transport...
    • Investigation of vesicle fusion in ER to Golgi transport.

    • Investigation of vesicle fusion in ER to Golgi transport.
    • Membrane proteins -- Physiological transport. ; Biological transport -- Regulation. ; Cell membranes. ; Golgi apparatus. ; Endoplasmic reticulum. ; Saccharomyces cerevisiae -- Cytology.
    • Many intracellular transport events are mediated by vesicles that bud from donor membranes and then fuse with acceptor membranes. The transport of secretory proteins between the ER and Golgi compartments of the yeast Saccharomyces cerevisiae has...
    • Protein spatial dynamics during homotypic vacuole fusion.

    • Protein spatial dynamics during homotypic vacuole fusion.
    • Membrane proteins -- Physiological transport. ; Membrane fusion. ; Tonoplasts. ; Biological transport -- Regulation. ; Saccharomyces cerevisiae -- Molecular aspects.
    • Membrane docking and fusion are essential steps in intracellular vesicular trafficking. The underlying molecular mechanisms are highly conserved between organisms. Yeast homotypic vacuole fusion has become a paradigm in studying this fundamental...
    • Mechanisms of Rab- and Rab effector-dependent membrane tethering, docking, and fusion.

    • Mechanisms of Rab- and Rab effector-dependent membrane tethering, docking, and fusion.
    • Membrane fusion. Membrane proteins -- Physiological transport. Yeast fungi -- Cytology. Medical Term Vacuoles -- metabolism. Cytoplasmic Vesicles -- metabolism rab GTP-Binding Proteins -- metabolism Proteolipids -- metabolism
    • Yeast vacuole fusion requires 4 SNAREs, the Rab GTPase Ypt7p, vacuolar lipids, the SNARE complex disassembly machinery Sec17p and Sec18p, and the heterohexameric HOPS complex. Vacuole fusion occurs in stages: cis-SNARE complexes (bound to one...

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