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Quantifying optical tomography methods for biomolecular sensing / by Jennifer-Lynn H. Demers.

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Author	Demers, Jennifer-Lynn H.
Title	Quantifying optical tomography methods for biomolecular sensing / by Jennifer-Lynn H. Demers.
Date	2014
Abstract	Diffuse optical imaging techniques can be combined with standard imaging technologies such as computed tomography (CT) and magnetic resonance (MR) imaging to allow measurement of spectroscopic signals pertaining to the molecular components of tissue. Some of the more promising approaches to image-guided spectroscopy rely upon inelastic scattering or fluorescence phenomena, which directly sample molecular contrast. Measurement of these molecular signal changes can then allow for diagnostic determination of tissue function or disease status in vivo. Molecular signal measurement often requires small tissue thickness and high signal intensity, yet when utilized with tomographic recovery it is often feasible to detect even lower signals at deep tissue depths. In this work, the range of useful signals and concentrations possible for molecular sensing with image-guidance are identified and key application areas examined. In order to better understand the advantages and disadvantages of each possible tomographic signal, a series of experiments were conducted in tissue phantoms as well as animal studies. Three imaging signals have been explored in detail: 1) Raman, 2) surface enhanced Raman and 3) molecular fluorescence. Previous work has shown, that with the combination of optical measurements and spatial information garnered from CT or MR scans, a higher level of contrast-to-background could be obtained. These gains in signal were verified for these imaging methods. Phantom measurements determined the concentration range necessary for a linear response for each signal and system pair. In-vivo and ex-vivo animal experiments provided data for a comparison of the signal strength and contrast with respect to the biologically relevant molecular contrast available. An understanding of the regions over which a linear response of the detector occurs for each optical contrast method can be used to guide experimental design, both in knowing the necessary level of contrast for successful tomographic imaging to occur, in order to then guide the dosing estimates for use of extrinsic contrasts and determine regions with sufficient Raman signal density for intrinsic contrast.
Description	xxiv, 189 leaves : ill. (mostly col.)
Format	application/pdf
College	Thayer School of Engineering.
Issuing Body	Dartmouth Dissertations are made available through the Dartmouth Digital Library.
Degree	Thesis (Ph.D.)--Dartmouth College.
Copyright	All rights reserved. No part of this book may be reproduced in any form by any electronic or mechanical means without permission in writing from the author.
DOI	http://dx.doi.org/10.1349/ddlp.1235

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