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Dendritic cell Fcγ receptor expression and function

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Call No.	QR185.8.F33 F36 1996
Author	Fanger, Neil A.
Title	Dendritic cell Fcγ receptor expression and function
Date	1996
Abstract	Three types of Fc receptors for IgG, FcyRI (CD64), FcyRII (CD32), and FcyRIII (CD 16) are expressed differentially on blood leukocytes. In particular, CD64 and CD32 are constitutively expressed on mononuclear phagocytes of the human myeloid system. Peripheral blood dendritic cells (DC), in contrast, have been reported to express CD32, but CD64 expression has been controversial. Studies were therefore conducted that confirmed that CD33b r iCD14n esHLA-DRb r iCD83P0 S circulating blood DC express CD64, although at a lower level than CD33b r iCD14b r iHLA-DRd i mCD83n eg blood monocytes. Following 2 days in culture, neither DC nor monocytes demonstrated any decrease in either CD64 or CD32 expression Stimulation with the cytokines IFN-Y and IL-10 increased CD64 expression on both DC and monocytes following 40 h stimulation, whereas GM-CSF and IL-12 had no effect on CD64 expression with either cell type. Interestingly, IL-4 decreased CD64 expression on monocytes, but had no effect on DC CD64 expression following 40 h stimulation. Subtle Fey receptor-mediated functional differences were also apparent between the DC and monocytes. Crosslinking of DC surface bound IgG resulted in no detectable superoxide anion release, whereas monocytes released substantial levels of superoxide anions under identical conditions. Moreover, CD64- and CD32-mediated phagocytosis of opsonized particles by the DC was lower than by the monocytes and correlated with lower DC expression levels of CD64 and CD32. Even with lower expression levels of CD64, however, the DC enhanced antigen presentation to a similar extent as the monocytes. Antigen targeted to CD64 expressed by either DC or monocytes was 10- to 1000-fold more potent then soluble protein for T cell activation. These studies suggest that both CD64 and CD32 play an integral role in DC uptake of opsonized particulate antigen during infection.
Description	xiv, 173 leaves : ill. (some col.)
Format	application/pdf
Advisor	Guyre, Paul M.
Subject	Fc receptors. Dendritic cells. Immune recognition.
College	Dartmouth Medical School -- Department of Physiology.
Issuing Body	Dartmouth Dissertations are made available through the Dartmouth Digital Library.
Degree	Thesis (Ph.D.)--Dartmouth College.
Copyright	All rights reserved. No part of this book may be reproduced in any form by any electronic or mechanical means without permission in writing from the author.
DOI	http://dx.doi.org/10.1349/ddlp.1500

Title	Page 1
Full Text	Dendritic Cell Fcy Receptor Expression and Function A Thesis Submitted to the Faculty in partial fulfillment of the requirements for the degree of Doctor of Philosophy in Physiology by Neil A. Fanger DARTMOUTH COLLEGE Hanover, New Hampshire May 1996

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Dendritic cell Fcγ receptor expression and function

Dendritic cell Fcγ receptor expression and function