Find results with:

Add another field

Search by date

to

Searching collections:

Dartmouth Dissertations

Add or remove collections

Home Dartmouth Dissertations nguyen2016 1

Reference URL

To link to the entire object, paste this link in email, IM or document

To embed the entire object, paste this HTML in website

To link to this page, paste this link in email, IM or document

To embed this page, paste this HTML in website

Mechanisms regulating interphase condensin II control of genome organization / by Huy Nguyen

you wish to report:

Your comment:

Your Name:

Submit

Cancel

...

Back to top

Powered by CONTENTdm®

Author	Nguyen, Huy
Title	Mechanisms regulating interphase condensin II control of genome organization / by Huy Nguyen
Date	2016
Abstract	How the genome is shaped directly influences how it is expressed. In fact, the genome is organized at many different levels, each imparting specific regulation over transcription. Fundamental principles regulating genome organization are poorly understood. More recently, a role for the chromosome structuring complex, condensin II, in organization of the genome has been identified. However, mechanisms regulating interphase condensin II are elusive. Herein this dissertation, I present data from experiments performed in Drosophila melanogaster identifying proteins involved in the loading and removal of condensin II from interphase chromosomes. First, by using yeast 2 hybrid assays, we identify that the chromodomain protein, Mrg15, interacts with condensin II subunit, Cap-H2, through its MRG binding motif. In cultured cells and Drosophila using subcellular fractionation, we show that Mrg15 recruits Cap-H2 to chromatin. Immunofluorescence and fluorescent in-situ hybridization (FISH) demonstrate that Mrg15 recruitment allows for condensin II’s chromosome compaction roles. Secondly, using an RNAi screen in cultured cells, we find that the SCF[superscript Slimb] complex (Slimb) regulates nuclear morphology through condensin II. We further show through immunoprecipitation that Slimb targets Cap-H2 for ubiqutination. This results in eviction of Cap-H2 from chromatin and repressed condensin II activity as demonstrated by immunofluorescence confirmation of inhibition of chromosome dispersal. Lastly, we identified that Casein Kinase I Alpha (CK1α) also functions to negatively regulate Cap-H2 and condensin II. Subcellular fractionation and immunofluorescent analysis of chromatin bound Cap-H2 reveals that CK1α normally evicts Cap-H2 from chromatin. Also, through cytological techniques, we find that CK1α influence over genome organization is mediated through condensin II regulation. In summary, we elucidate condensin II regulation during interphase by identifying three previously unknown condinsin II interactors: Mrg15, Slimb, and CK1α. We end by describing a role for CK1α regulation of condensin II during the response to heat stress in Drosophila. These findings are impactful in that they further our limited knowledge of genome organization. Through a better understanding of genome organization, we can better understand mechanisms regulating transcription and with that, better understand biology.
Description	1 online resource (xv, 358 leaves) : illustrations (chiefly color)
Format	application/pdf
College	Geisel School of Medicine at Dartmouth -- Department of Genetics.
Issuing Body	Dartmouth Dissertations are made available through the Dartmouth Digital Library.
Degree	Thesis (Ph.D)--Dartmouth College.
Copyright	Made available by the author under a Creative Commons Attribution-NonCommercial License.; Made available by the Trustees of Dartmouth College under a Creative Commons Attribution-NonCommercial License. https://creativecommons.org/licenses/by-nc/4.0/
DOI	https://doi.org/10.1349/ddlp.2195

Find results with:

Searching collections:

Home Dartmouth Dissertations nguyen2016 1

Mechanisms regulating interphase condensin II control of genome organization / by Huy Nguyen

Object Description

Description

Mechanisms regulating interphase condensin II control of...

Mechanisms regulating interphase condensin II control of...

	All
	Pages Enter page numbers or page ranges separated by commas. For example: 1,3,5-12