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Characterization of two Saccharamyces cerevisiae genes involved in mRNA export.

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Call No.	QP623.5.M47 D45 1997
Author	Del Priore, Andrea Verónica
Title	Characterization of two Saccharamyces cerevisiae genes involved in mRNA export.
Date	1997
Abstract	I report the further characterization of an essential S. cerevisiae nucleoporin, RAT7/NUP159, shown previously to be involved in mRNA export. The Rat7p/Nup159p can be divided into three domains: an N-terminal domain, a central repeat domain, and a C-terminal domain. I performed a detailed structural-functional analysis of the Rat7p/Nup 159p. The putative coiled-coil regions, located at the carboxyl-terminus of the protein, were found to be essential. The repeat region was completely dispensable for growth. Deletion of the amino-terminal third of the protein or the C-terminal portion between the repeat region and the coiled-coils resulted in temperature-sensitivity. The two alleles showed distinct phenotypes with respect to clustering of nuclear pore complexes (NPCs) and the location of Rat7p/Nup 159p following a shift to 37ºC. Taken together, my data suggest that Rat7p/Nup159p is anchored within the NPC through its coiled-coil region and adjacent sequences. In addition, I postulate that the N-terminal third of Rat7p/Nup159p either plays a direct role in mRNA export or interacts with other nucleoporins that play a direct role. I also report the isolation of a new gene, called RSS1, through a high copy suppressor screen using the rat7-1 temperature sensitive allele. Rss1p encodes a novel protein of 538 amino acids which contains an extended predicted coiled-coil domain and is located both at NPCs and in the cytoplasm. Overexpression of Rss1p partially suppressed all the defects associated with the rat7-1 allele at the non-permissive temperature of 37ºC. Depletion of Rss1p led to cessation of growth and nuclear accumulation of poly(A)+ RNA without affecting nuclear protein import or nuclear pore complex distribution, suggesting that RSS1 is directly involved in mRNA export. Because both rat7-1 cells and cells depleted for Rss1p are defective in mRNA export, my data is consistent with both gene products playing essential roles in the process of mRNA export and suggest that Rss1p overexpression suppresses the growth defect of rat7-1 cells at 37ºC by acting to maintain mRNA export.
Description	1 online resource (xiv, 167 leaves) : illustrations
Format	application/pdf
Advisor	Barlowe, Charles
Subject	Messenger RNA -- Physiological transport. ; Saccharomyces cerevisiae -- Genetics. ; Proteins -- Synthesis -- Genetic aspects.
College	Dartmouth College -- Department of Biochemistry.
Issuing Body	Dartmouth Dissertations are made available through the Dartmouth Digital Library.
Degree	Thesis (Ph.D)--Dartmouth College.
Copyright	In Copyright. This material is protected by copyright and/or related rights. You are free to use this material in any way that is permitted by copyright law that applies to your use. For other uses, you need to obtain permission from the rights-holder(s). http://rightsstatements.org/page/InC/1.0/
DOI	https://doi.org/10.1349/ddlp.2700

Title	delpriore1997 1
Full Text	Characterization of Two Saccharomyces cerevisiae Genes Involved in mRNA Export A thesis submitted to the faculty in partial fulfillment of the requirements for the degree of Doctor of Philosophy in Biochemistry by Andrea Veronica Del Priore Dartmouth College Hanover, New Hampshire May 26, 1997

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Characterization of two Saccharamyces cerevisiae genes involved in mRNA export.

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