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Differential MCL1 phosphorylation occurs through distinct pathways.

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Call No.	RC268.43 .D66 2001
Author	Domina, Aaron Mark
Title	Differential MCL1 phosphorylation occurs through distinct pathways.
Date	2001
Abstract	Protein kinase C activators and microtubule damaging drugs stimulate BCL2 phosphorylation, which has been associated with either enhancement or inhibition of cell viability. In a Burkitt lymphoma cell line, both types of agents likewise stimulated phosphorylation of myeloid cell leukemia 1 (MCL1), another viability-promoting BCL2 family member. However, while MCL1 phosphorylation induced by the protein kinase C activator, 12-O-tetradecanoylphorbol 13-acetate (TPA), did not affect its electrophoretic mobility, microtubule damaging agents, such as taxol, induced MCL1 phosphorylation associated with a band shift to decreased mobility. Inhibitors of extracellular signal-regulated kinase (ERK) activation blocked TPA-induced MCL1 phosphorylation, but not the taxol-induced band shift. TPA-induced MCL1 phosphorylation occurred rapidly and was not associated with decreased viability, while the taxol-induced band shift occurred upon extended exposure as cells accumulated in G 2 /M followed by cell death. Protein phosphatase 1/2a inhibitors also induced the MCL1 band shift/phosphorylation. Further investigation by two-dimensional phosphopeptide mapping and mutational analysis revealed that MCL1 was differentially phosphorylated by these various agents. Thus, MCL1 undergoes distinct types of phosphorylation: (i) TPA-induced, ERK-associated phosphorylation, which does not alter the electrophoretic mobility of MCL1, and (ii) ERK-independent phosphorylation, which results in an MCL1 band shift and is induced by events in G2/M or protein phosphatase 1/2a inhibitors.
Description	1 online resource (xiii, 149 leaves) : illustrations (some color)
Format	application/pdf
Advisor	Craig, Ruth W.
Subject	Myeloid leukemia -- Genetic aspects. ; Phosphorylation. ; Antioncogenes. ; Cancer cells -- Growth -- Regulation. ; Immune response -- Regulation.
College	Dartmouth Medical School -- Department of Pharmacology and Toxicology.
Issuing Body	Dartmouth Dissertations are made available through the Dartmouth Digital Library.
Degree	Thesis (Ph.D)--Dartmouth College.
Copyright	In Copyright. This material is protected by copyright and/or related rights. You are free to use this material in any way that is permitted by copyright law that applies to your use. For other uses, you need to obtain permission from the rights-holder(s). http://rightsstatements.org/page/InC/1.0/
DOI	https://doi.org/10.1349/ddlp.3058

Title	domina2001 1
Full Text	DIFFERENTIAL MCL1 PHOSPHORYLATION OCCURS THROUGH DISTINCT PATHWAYS A Thesis Submitted to the Faculty in partial fulfillment of the requirements for the degree of Doctor of Philosophy tn Pharmacology and Toxicology by Aaron Mark Domina DARTMOUTH COLLEGE Hanover, New Hampshire March 14,2001 Reproduced with permission of the copyright owner. Further reproduction prohibited without permission.

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