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Light responses and photoadaptation in Neurospora crassa.

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Call No.	Ph.D. 2010
Author	Chen, Chen-Hui.
Title	Light responses and photoadaptation in Neurospora crassa.
Date	2010
Abstract	WC-1 and WC-2 are essential for most light responses in Neurospora crassa, but the molecular mechanisms underlying gene induction and the roles of other real and putative photoreceptors remain poorly characterized. Unsupervised hierarchical clustering of genome-wide microarrays reveals 5.6% of detectable transcripts, including several novel mediators, that are either early or late light-responsive. White Collar Complex (WCC) regulates both types of light responses. Light-responsive Submerged Protoperithecia-1 (SUB-1), a GATA family transcription factor, is essential for most late light responses. Following a 15-minute light stimulus, the WCC directly binds the sub-1 promoter. Bioinformatics analysis detects many Early Light Response Elements (ELREs), as well as Late Light Response Elements (LLREs). These data provide a global picture of the transcriptional response to light, as well as illuminating the cis and trans-acting elements comprising the regulatory signaling cascade that governs the photobiological response. Photoadaptation, the ability to attenuate a light response upon prolonged light exposure while remaining sensitive to escalating changes in light intensity, is essential for organisms to appropriately decipher time information, yet the underlying molecular mechanisms are poorly understood. In Neurospora crassa, VVD, a small LOV domain containing photoreceptor protein, is responsible for most photoadaptation responses. We report that there is a physical interaction between VVD and the WCC. Using two previously characterized VVD mutants, we show that the level of interaction is correlated with the level of WCC repression in constant light, and that even light-insensitive VVD is sufficient to regulate photoadaptation in vivo. We provide evidence that a functional GFP-VVD fusion protein accumulates in the nucleus upon light induction but that nuclear localization of VVD does not require light. Constitutively expressed VVD alone is sufficient to change the dynamics of photoadaptation. Thus, our results demonstrate a direct molecular connection between two of the most essential light signaling components in Neurospora, VVD and WCC, illuminating a previously uncharacterized process for light-sensitive eukaryotic cells.
Description	xi, 188 leaves : ill. (some col.) ; 28 cm.
Format	application/pdf
Advisor	Jennifer J Loros C. Robertson McClung, Jay C Dunlap, Michael L Whitfield, David E Somers
Subject	Neurospora crassa -- Effect of light on. Neurospora crassa -- Genetics. Neurospora crassa -- Physiology. Neurospora crassa -- Molecular aspects. Genetic transcription -- Regulation.
College	Dartmouth Medical School -- Department of Genetics.
Issuing Body	Dartmouth Dissertations are made available through the Dartmouth Digital Library.
Degree	Thesis (Ph.D.)--Dartmouth College.
Copyright	All rights reserved. No part of this book may be reproduced in any form by any electronic or mechanical means without permission in writing from the author.
DOI	http://dx.doi.org/10.1349/ddlp.380

Title	Page 1
Format	application/pdf
Issuing Body	Dartmouth Dissertations are made available through the Dartmouth Digital Library.
Degree	Thesis (Ph.D.)--Dartmouth College.
Full Text	Light responses and photoadaptation in Neurospora crassa A Thesis Submitted to the Faculty in partial fulfillment of the requirements for the degree of Doctor of Philosophy in Genetics by Chen-Hui Chen DARTMOUTH COLLEGE Hanover, New Hampshire May, 2010 Examining Committee: ________________________ (chair) C. Robertson McClung ________________________ Jay C. Dunlap ________________________ Jennifer J. Loros ________________________ ________________________ Michael L. Whitfield Brian W. Pogue, Ph.D. ________________________ Dean of Graduate Studies David E. Somers

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Light responses and photoadaptation in Neurospora crassa.

Light responses and photoadaptation in Neurospora crassa.