Find results with:

Add another field

Search by date

to

Searching collections:

Dartmouth Dissertations

Add or remove collections

Home Dartmouth Dissertations Page 1

Reference URL

To link to the entire object, paste this link in email, IM or document

To embed the entire object, paste this HTML in website

To link to this page, paste this link in email, IM or document

To embed this page, paste this HTML in website

Phospho-regulation of the kinesin-13 Kif2b ensures error-free mitosis

you wish to report:

Your comment:

Your Name:

Submit

Cancel

...

Back to top

Powered by CONTENTdm®

Author	Hood, Emily A.
Title	Phospho-regulation of the kinesin-13 Kif2b ensures error-free mitosis
Date	2012
Abstract	Solid tumors are frequently aneuploid and many display high rates of ongoing chromosome mis-segregation in a phenomenon called chromosomal instability (CIN). The most common cause of CIN is the persistence of aberrant kinetochore-microtubule (k-MT) attachments that manifest as lagging chromosomes in anaphase. K-MT attachment errors form during prometaphase due to stochastic interactions between kinetochores and microtubules. The kinesin-13 protein Kif2b promotes the correction of k-MT attachment errors in prometaphase, but the mechanism restricting this activity to prometaphase remains unknown. Using mass spectrometry we have identified multiple phosphorylation sites on Kif2b, some of which are acutely sensitive to inhibition of Polo-like kinase 1 (Plk1). We show that Plk1 directly phosphorylates Kif2b at threonine 125 (T125) and serine 204 (S204), and that these two sites differentially regulate Kif2b function. Phosphorylation of S204 is required for the kinetochore localization and activity of Kif2b in prometaphase, and phosphorylation of T125 is required for Kif2b activity in the correction of k-MT attachment errors. These data demonstrate that Plk1 regulates both the localization and activity of Kif2b during mitosis to promote the correction of k-MT attachment errors to ensure mitotic fidelity.
Description	viii, 146 leaves : ill. (some col.) ; 28 cm.+ 1 computer optical disc (4 3/4 in.)
Format	application/pdf
Advisor	Compton, Duane A; Gladfelter, Amy; Smith, Elizabeth; Ohi, Ryoma
College	Dartmouth Medical School -- Department of Biochemistry.
Issuing Body	Dartmouth Dissertations are made available through the Dartmouth Digital Library.
Degree	Thesis (Ph.D.)--Dartmouth College.
Copyright	All rights reserved. No part of this book may be reproduced in any form by any electronic or mechanical means without permission in writing from the author.
DOI	http://dx.doi.org/10.1349/ddlp.428

Title	Page 1
Format	application/pdf
Issuing Body	Dartmouth Dissertations are made available through the Dartmouth Digital Library.
Degree	Thesis (Ph.D.)--Dartmouth College.
Full Text	PHOSPHO-REGULATION OF THE KINESIN-13 KIF2B ENSURES ERROR-FREE MITOSIS A Thesis Submitted to the Faculty in partial fulfillment of the requirements for the degree of Doctor of Philosophy in Biochemistry by Emily A. Hood DARTMOUTH COLLEGE Hanover, New Hampshire 23rd May 2012 Examining Committee ________________________ (chair) Duane A. Compton ________________________ Amy Gladfelter ________________________ Elizabeth Smith ________________________ Ryoma Ohi ________________________ Brian W. Pogue, PhD Dean of Graduate Studies

Find results with:

Searching collections:

Home Dartmouth Dissertations Page 1

Phospho-regulation of the kinesin-13 Kif2b ensures error-free mitosis

Object Description

Description

Phospho-regulation of the kinesin-13 Kif2b ensures...

Phospho-regulation of the kinesin-13 Kif2b ensures...

	All
	Pages Enter page numbers or page ranges separated by commas. For example: 1,3,5-12