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Natural and engineered metabolism of Thermoanaerobacterium saccharolyticum during growth on plant derived mono- and polysaccharides / by Devin Hedley Currie.

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Author	Currie, Devin Hedley.
Title	Natural and engineered metabolism of Thermoanaerobacterium saccharolyticum during growth on plant derived mono- and polysaccharides / by Devin Hedley Currie.
Date	2013
Abstract	Many microbes thrive in conditions that would be detrimental to human life. These microorganisms have been dubbed extremophiles. Of these, members of a subcategory, termed thermophiles, grow optimally above 45°C. Thermoanaerobacterium saccharolyticum is, as suggested by its name, a thermophilic, anaerobic bacterium endowed with a diverse repertoire of metabolic abilities and considerable industrial potential. Capable of rapid growth on various plant cell wall components, T. saccharolyticum is an ideal candidate in which to study thermophilic polysaccharide deconstruction and fermentation. This thesis details the study of T. saccharolyticum and its relationship with plant biomass. T. saccharolyticum has been studied since early 1990s. Despite this, much of its basic biology remains unexplored. Here, we attempted a large scale collaborative study of T. saccharolyticum was undertaken with the goal of investigating genomic, transcriptomic, and metabolic aspects of the organism. As a result, we are able to put forward a broad description of T. saccharolyticum's physiology which will aid future researchers wishing to use T. saccharolyticum for the production of ethanol or other products from complex and somewhat toxic substrates. Following this, secreted and transmembrane proteins were characterized using proteomic and transcriptomic analysis of cultures grown on glucose, starch, xylose and xylan. Many differentially expressed enzymes and transporters were identified including a number of putative ABC-tranporters and T. saccharolyticum's S-layer protein. Next, it was shown that T. saccharolyticum, while not natively capable of crystalline cellulose hydrolysis, can serve as a platform for the hereologous expression of components of the cellulose active enzyme complex termed the cellulosome, specifically that of C. thermocellum. Finally, we present data on the characterization of a protein bound organelle, or bacterial microcompartment, present in T. saccharolyticum, the first from a thermophile. This microcomp
Description	xxi, 281 leaves : ill. (some col.) 28 cm.
Format	application/pdf
Advisor	Lee R. Lynd, George A. O'Toole, Karl E. Griswold, Michael W. W. Adams
College	Dartmouth College. Department of Biology.
Issuing Body	Dartmouth Dissertations are made available through the Dartmouth Digital Library.
Degree	Thesis (Ph.D.)--Dartmouth College.
Copyright	All rights reserved. No part of this book may be reproduced in any form by any electronic or mechanical means without permission in writing from the author.
DOI	http://dx.doi.org/10.1349/ddlp.951

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Natural and engineered metabolism of Thermoanaerobacterium saccharolyticum during growth on plant derived mono- and polysaccharides / by Devin Hedley Currie.

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