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Role of Whi3 protein aggregation behavior in cell-cycle regulation and cell polarity / by ChangHwan Lee.

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Author	Lee, Chang-Hwan.
Title	Role of Whi3 protein aggregation behavior in cell-cycle regulation and cell polarity / by ChangHwan Lee.
Date	2013
Abstract	Whi3 is a G1/S regulator and known to inhibit G1 CDK activity. It was first described as a cell-size control gene in a set of cell division cycle mutants in Saccharomyces cerevisiae. Dysfunction or deletion of Whi3 leads to abnormal cell cycle control and morphogenesis. Whi3 contains two conserved and interesting domains: a RRM domain which binds a large set of mRNAs, and a polyQ tract, which promotes protein aggregation. Although it is evident that these functions of Whi3 are tightly linked to many cellular functions such as cell cycle control or cell polarization, the basis, precise mechanisms and the role of Whi3 remain unknown. This thesis addresses some of the questions about the roles of Whi3 in cell functionality. How is cell cycle progression controlled through Whi3? What are the functions of the RRM and polyQ tract in Whi3 for the timing variability in cell cycle? How do the transcripts bound to Whi3 localize and why is it important for cell cycle control or cell polarization? Are there any other cellular function that are modulated by Whi3? The studies presented in this thesis demonstrate an unprecedented role for stabilization of G1 cyclin mRNA through Whi3 aggregation, as well as clustering of G1 cyclin mRNA. We also reveal novel functions of Whi3 aggregation and RNA binding in cell polarization, especially in polarized cell growth, morphogenesis and actin polarization. In this thesis, many novel microscopic, biochemical and bioinformatical techniques are described using Ashbya gossypii, which is a multinucleated and filamentous fungus. Ashbya is a powerful system to study timing variability in cell cycle and cell polarization due to multiple nuclear division in a common cytoplasm and the frequent maintenance and establishment of cell polarity in a cell regardless of cell cycle. Overall, this thesis describes new and multiple roles of Whi3 for cell cycle control and cell polarization.
Description	xi, 175 leaves : ill. (some col.) 28 cm. + 1 computer optical disc (4 3/4 in.)
Format	application/pdf
Advisor	Amy S. Gladfelter, Sharon E. Bickel, Charles N. Cole, Heather True
College	Dartmouth College. Department of Biological Sciences.
Issuing Body	Dartmouth Dissertations are made available through the Dartmouth Digital Library.
Degree	Thesis (Ph.D.)--Dartmouth College.
Copyright	All rights reserved. No part of this book may be reproduced in any form by any electronic or mechanical means without permission in writing from the author.
DOI	http://dx.doi.org/10.1349/ddlp.981

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