Dartmouth Digital Library Program

Thumbnail

Title

Subject

Description

Collection

Characterization of transporters involved in uptake of choline and other trimethylamine-containing compounds in the opportunistic pathogen Pseudomonas aeruginosa / by Adel A. Malek.
Tumors -- Treatment -- Methodology.;""Nanoparticles -- Therapeutic use."";""Cell receptors."";""Antigenic determinants."";""Neoplasms -- therapy."";""Nanotechnology -- methods."";""Epitopes.""
Dartmouth Dissertations

Computational methods for the discovery and application of protein sequence-structure-function relationships / by Lu He.
Proteins are ubiquitous in cells and are essential to a wide range of biological processes. Since existing proteins occupy only a small portion of the space of possible amino acid composition, understanding their sequence-structure-function...
Dartmouth Dissertations

Structural characterization of the LysR-type virulence factor, AphB, and insight into its mehcanism of transcriptional activation at the tcpPH promoter in Vibrio cholerae
The LysR-type virulence regulator, AphB, initiates the Vibrio cholerae virulence cascade resulting in expression of the two critical virulence factors, toxin-coregulated pilus and cholera toxin, by activating transcription at the tcpPH promoter....
Dartmouth Dissertations

Determinants of competitive outcome between rotifers and cladocerans.
Cladocera. ; Rotifera. ; Competition (Biology).
Dartmouth Dissertations

Molecular characterization of the TCP biogenesis apparatus in Vibrio cholerae.
Vibrio cholerae -- Molecular genetics. Pili (Microbiology). Cholera -- Pathogenesis. Proteins -- Secretion.
Vibrio cholerae is a water-borne, Gram negative enteropathogen that causes the gastrointestinal disorder, cholera, in humans. A critical step in cholera pathogenesis is the attachment and colonization of intestinal cells, and the formation of...
Dartmouth Dissertations

The effect of suspended sediments on planktonic rotifers and cladocerans.
Freshwater zooplankton -- Effect of sediments on. Rotifera. Cladocera.
Dartmouth Dissertations

Specificity in signaling : bacterial decision-making through the cyclic diguanylate second messenger / by Kurt M. Dahlstrom
Cyclic diguanylate (c-di-GMP) is a primarily bacterial and nearly universal second messenger used to stimulate a variety of responses from the cell by binding effector proteins. It is created by enzymes called diguanylate cyclases (DGCs) and broken...
Dartmouth Dissertations

Structure based drug discovery of potent and specific antimicrobial agents for anthrax.
Bacillus cereus. Anti-infective agents -- Design. Enzyme inhibitors -- Therapeutic use. Enzyme inhibitors -- Structure-activity relationships. Folic acid -- Antagonists. Anthrax -- Treatment. Tetrahydrofolate Dehydrogenase.
Infections by pathogenenic microbes are an increasing problem due to a lack of universal vaccines and growing resistance to current antibiotics. The constant battle for researchers in the fight against pathogens is to design new and improved drugs...
Dartmouth Dissertations

NKG2D-ligand mediated immune interactions in the human female reproductive tract.
Killer cells. Natural immunity. Major histocompatibility complex. Generative organs.
The immune system in the human endometrium maintains the delicate balance between defense against foreign pathogens and tolerance allowing for the implantation and development of a semi-allogeneic conceptus. Uterine NK cells which may comprise up...
Dartmouth Dissertations

Regulation of innate immunity of mouse uterine epithelial cells by keratinocyte growth factor and estradiol.
Epithelial cells. Uterus -- Physiology. Keratinocytes -- Physiological effect. Estradiol -- Physiological effect. Growth factors -- Physiological effect. Chemokines.
The mucosal immune system of the female reproductive tract (FRT) balances the dual functions of protection against invading pathogens and adaptation of the endometrial environment for successful fertilization and implantation. Growth factors are...
Dartmouth Dissertations

Regulation of the haptoglobin-hemoglobin scavenger receptor CD163 in the inflammatory response to endotoxin.
CD antigens. Cell receptors. Endotoxins -- Pathophysiology. Glycoproteins -- Pathophysiology. Immune response -- Regulation. Septicemia -- Molecular aspects. Macrophages. Haptoglobins. Hemoglobin. Receptors
Sepsis is a worldwide problem with an annual mortality rate reaching as high as 45%. Understanding its pathogenesis and the innate immune response to infection is key to the development of new treatments. CD163, a monocyte/macrophage-specific...
Dartmouth Dissertations

Genetic regulation of PBP2 and PBP4 in community-acquired methicillin-resistant Staphylococcus aureus
Staphylococcus aureus contains 4 native, membrane-bound penicillin-binding proteins (PBP1-4) which are responsible for critical peptidoglycan transglycosylation and transpeptidation reactions in the cell wall. Additionally, PBP2 and PBP4 also...
Dartmouth Dissertations

Site-specific modification of glycoproteins expressed in an engineered strain of Escherichia coli.
Glycoproteins -- Synthesis. Escherichia coli -- Biotechnology. Bioconjugates -- Synthesis. Protein engineering.
We have created an E. coli based platform for protein modification by coupling an engineered glycosylation pathway with a subsequent chemoenzymatic labeling strategy in which the glycans are oxidized to provide a bioorthogonal aldehyde group that...
Dartmouth Dissertations

Interrogation of autolysins as a source of novel bacteriolytic agents / by Daniel Carmen Osipovitch
Staphylococcus aureus is a formidable pathogen, and its high morbidity and mortality rates are compounded by its ability to quickly develop antibiotic resistance. The current antimicrobial pipeline is inadequate to curb this mounting public health...
Dartmouth Dissertations

Metal distribution in Arabidopsis.
Arabidopsis -- Effect of metals on. ; Zinc -- Physiological transport. ; Iron -- Physiological transport. ; Plants -- Effect of iron on. ; Plants -- Effect of zinc on.
Iron and zinc deficiency commonly limit plant growth and crop yields. Furthermore, deficiencies in both of these micronutrients are common throughout the world, particularly in developing countries where people may only consume simple diets...
Dartmouth Dissertations

Analyses of SOS-type DNA repair processes in mammalian cells
DNA repair. ; Mammals -- Cytology. ; Mutagenesis
Dartmouth Dissertations

The effect of iron, oxygen, and heme on the expression of Bradyrhizobium japonicum hemA gene.
Rhizobium -- Genetics. ; Heme -- Genetics. ; Bacterial genetics. ; Genetic regulation.
Dartmouth Dissertations

Conversion of paper sludge to ethanol and analysis of selection based strain improvement for recombinant Saccharomyces cerevisiae expressing saccharolytic enzymes through continuous culture.
Cellulase -- Biotechnology. ; Hydrolysis. ; Paper industry -- Technological innovations. ; Biomass energy -- Economic aspects. ; Saccharomyces cerevisiae -- Genetics. ; Enzymes -- Industrial applications.
Paper sludge is the largest solid waste stream produced by pulp and paper industry, and is also an attractive feedstock for emergent technologies based on processing of cellulosic biomass featuring enzymatic hydrolysis. This study focuses on...
Dartmouth Dissertations

Expression and recovery of recombinant proteins in a novel high cell density prokaryotic system based on Ralstonia eutropha.
Ralstonia -- Biotechnology. Microbial biotechnology. Industrial microbiology. Recombinant proteins -- Synthesis. Recombinant Proteins -- Biosynthesis.
Escherichia coli is the standard host for recombinant protein expression in bacteria, but suffers from some limitations (e.g. poor fermentation performance, inclusion body formation and proteolysis). A novel recombinant protein expression system,...
Dartmouth Dissertations

Novel levels of Ras1 regulation in the human fungal pathogen, Candida albicans / by Amy Elizabeth Piispanen.
Neurotrophic functions.;""Growth factors."";""Exercise -- Physiological aspects."";""Cognition -- Effect of exercise on."";""Cognition -- physiology.""
Dartmouth Dissertations

Mechanisms of a glial modulating agent, propentofylline :potential new treatment for glioblastoma multiforme
Glioblastoma multiforme is the most common and aggressive primary brain tumor with a very poor prognosis despite multi-modalities of treatment. As a result, there is a critical need to develop alternative therapies. Propentofylline (PPF) is a...
Dartmouth Dissertations

From target discovery to high-throughput screens--selective CAL PDZ inhibitors as candidate CF therapeutics
Peptides -- Therapeutic use. ; Chloride channels -- Pathophysiology. ; Ligands (Biochemistry) ; Protein-protein interactions. ; Cystic fibrosis -- Molecular aspects. ; High throughput screening (Drug development) ; PDZ Domains. ; Cystic Fibrosis...
PDZ domains are protein:protein interaction domains that serve as molecular scaffolds, especially in the case of specialized cellular environments like polarized airway epithelial cells of the human lung. Historically the domains have been thought...
Dartmouth Dissertations

Uterine epithelial cell release of MIP3, TNF and TGF in response to Escherichia coli, Lactobicillus rhamnosus and pathogen associated molecular patterns (PAMP) and the influence of estradiol on cytokine release.
Epithelial cells. Estradiol -- Physiological effect. Tumor necrosis factor. Transforming growth factors-beta. Cytokines -- Pathophysiology. Escherichia coli. Lactobacillus rhamnosus. Uterus -- Pathophysiology.
The research presented in this thesis tested the hypothesis that uterine epithelial cells (UEC) are selectively responsive to bacteria and pathogen associated molecular patterns (PAMP) and that responses are influenced by estradiol (E 2 ). We...
Dartmouth Dissertations

Thermodynamic studies of copper binding to azurin : development and application of a novel method to determine the reduction potential and reduction thermodynamics of metalloproteins / by Molly L. Croteau
Cupredoxins, such as stellacyanin, plastocyanin, amicyanin, rusticyanin, and azurin perform electron transfer (ET) reactions with partner protein(s) in the cell. The role of the redox cofactor in these proteins is played by a copper ion, which...
Dartmouth Dissertations

The Vibrio cholerae AraC-like protein ToxT: activation of tcpA expression and domain organization.
Vibrio cholerae -- Molecular genetics. Genetic transcription -- Regulation. Promoters (Genetics). DNA-binding proteins.
ToxT is a member of the AraC/XylS family of transcriptional regulators, and functions to activate expression of virulence genes in the intestinal pathogen Vibrio cholerae. The aim of this research was to investigate the mechanism of ToxT-dependent...
Dartmouth Dissertations

The structure and stability of a freshwater pelagic community.
Lake ecology -- Vermont -- Mitchell, Lake. ; Algae -- Control. ; Daphnia.
Dartmouth Dissertations

The role of TonB-dependent receptors and alternate sigma factors in the Bradyrhizobium japonicum/soybean symbiosis.
Rhizobium -- Genetics. Cell receptors. Transcription factors. Bacteria.
Bradyrhizobium japonicum forms a nitrogen-fixing symbiosis with soybean. Signaling events, such as production of flavonoids and Nod factor, initiate the interaction, leading to formation of specialized root organs called nodules. A B. japonicum...
Dartmouth Dissertations

Virulence and motility gene regulation in Vibrio cholerae.
Vibrio cholerae -- Molecular genetics. DNA-binding proteins. Genetic transcription -- Regulation. Promoters (Genetics)
The ctx and tcp genes that encode cholera toxin and the toxin co-regulated pilus, the major virulence determinants of Vibrio cholerae, are repressed by the nucleoid-associated protein H-NS and activated by the AraC-like transcriptional regulator...
Dartmouth Dissertations

Characterization of factors critical for initial stages of Vibrio cholerae intestinal colonization.
Vibrio cholerae -- Molecular genetics. ; Bacteria -- Adhesion. ; Virulence (Microbiology) -- Molecular aspects. ; Pili (Microbiology). ; DNA-binding proteins. ; Cholera -- Pathogenesis.
Vibrio cholerae is the etiologic agent of cholera in humans. Intestinal colonization occurs via ingestion of contaminated food and water and results in symptoms that include the production of the characteristic rice water stool, vomiting, and...
Dartmouth Dissertations

Chimeric NKG2D receptor expressing T cells as immunotherapy for cancer.
T cells -- Receptors. Killer cells. Cell-mediated cytotoxicity. Cancer -- Immunotherapy.
Despite advances in standard treatment options such as surgery, chemotherapy, and radiation, cancer remains the second leading cause of death in the United States. Moreover, many current therapies result in adverse effects. Therefore the...
Dartmouth Dissertations

c-di-GMP mediated regulation of the LapA biofilm adhesin through proteolysis by the calcium-dependent cysteine protease LapG / by Chelsea D. Boyd.
The localization of the putative adhesin LapA to the cell surface is a key regulatory step required by Pseudomonas fluorescens Pf0-1 to irreversibly attach to a surface and form a biofilm. LapA contains several domains conserved in predicted...
Dartmouth Dissertations

The Pseudomonas fluorescens pho regulon and its role in modulating biofilm formation in response to environmental cues.
Pseudomonas fluorescens -- Genetics. Attachment mechanisms (Biology) Biofilms -- Growth. Phosphates -- Physiological effect. Medical Term: Biofilms -- growth and development. Adhesins, Bacterial.
Microbial biofilm formation is thought of as a developmental pathway, whereby cells progress through environmentally regulated and temporally distinct stages during transition from free-swimming lifestyles to members of a structured...
Dartmouth Dissertations

Endogenous leukocyte activation by chimeric antigen receptor T cell immunotherapy / by Paul Spear.
Many protocols for adoptive T cell therapy (ACT) include preparative conditioning strategies to deplete host lymphocytes prior to T cell infusion. Total body irradiation and high-dose chemotherapy regimens not only relieve immunosuppression, but...
Dartmouth Dissertations

Identification of novel proteins involved in Vibrio cholerae motility.
Vibrio cholerae. Bacteria -- Motility. Flagella (Microbiology) -- Physiology. Membrane proteins.
Dartmouth Dissertations

Chemical ecology : bacterial phenazines as toxins and paracrine signals in Candida albicans / by Diana K. Morales Gamba.
Supernovae -- Observations.;""Supernova remnants -- Observations."";""Supernovae -- Spectra.""
Dartmouth Dissertations

The site-specific modification of therapeutic proteins produced in Escherichia coli : implementation of a combinatorial bispecific antibody fragment library against human breast carcinomas
Immunoglobulins -- Synthesis. -- Escherichia coli -- Biotechnology -- Protein engineering -- Breast -- Cancer -- Immunotherapy.
The ability to site-specifically modify therapeutic proteins for the attachment of pharmacokinetic-enhancing molecules is the principal goal of many research programs. Site-specific modalities are often introduced to provide an additional...
Dartmouth Dissertations

Temperature effects on food web interactions : from lakes to landscapes / by Samuel Butler Fey.
Temperature has powerful effects on all scales of biological organization from biochemical reactions to ecosystem processes. Despite the well-appreciated influence and thorough understanding of the physiological and population consequences of...
Dartmouth Dissertations

Substrate recognition by the phenylalanine-adenylating domain of gramicidin synthetase, and redesign of nonribosomal peptide synthetases by modulation of substrate specificity.
Peptides -- Synthesis -- Mathematical models. ; Peptides -- Physiological effect. ; Peptides -- Biotechnology. ; Gramicidins. ; Immunospecificity. ; Site-specific mutagenesis. ; Binding sites (Biochemistry). ; Phenylalanine. ; Peptide Biosynthesis.
Non-ribosomal peptide synthetases (NRPS) are a family of enzymes that assemble a variety of pharmacologically interesting polypeptides from canonical and non-canonical amino acids. The identity and connectivity of the monomers in the final product...
Dartmouth Dissertations

Structural studies of Vibrio cholerae virulence gene regulators.
Vibrio cholerae -- Molecular genetics. ; Virulence (Microbiology) -- Molecular aspects. ; Genetic regulation. ; Viral proteins.
Cholera is a diarrheal disease caused by the Gram-negative bacterium Vibrio cholerae. The disease is acquired by oral ingestion of contaminated water or food. In order for Vibrio cholerae to cause the disease cholera, it must produce two important...
Dartmouth Dissertations

Characterization of type IVb pilus components essential for functional TCP of Vibrio cholerae O395, including implications on the hierarchy of microcolony formation and virulence / by Jarrad Matthew Marles.
Vibrio cholerae -- Molecular genetics. ; Pili (Microbiology). ; Virulence (Microbiology) -- Molecular aspects. ; Fimbriae Proteins.
Vibrio cholerae is the etiological agent of the acute intestinal disorder cholera. The source of cholera infection is typically fecal contamination of potable water sources. V. cholerae is able to colonize the host intestine and trigger the onset...
Dartmouth Dissertations

Mechanisms of survival and persistence of Vibrio cholerae.
Vibrio cholerae -- Molecular genetics. ; Pili (Microbiology). ; Proteins -- Secretion. Cholera -- Pathogenesis.
Vibrio cholerae is the etiologic agent of the severe diarrheal disease cholera. Colonization of the host environment by V. cholerae requires the type IV toxin co-regulated pilus (TCP). Adequate colonization by V. cholerae is essential for cholera...
Dartmouth Dissertations

A novel high-cell-density recombinant protein production system based on Ralstonia eutropha.
Recombinant proteins -- Synthesis. Ralstonia. Alcaligenes eutrophus. Organophosphorus compounds -- Synthesis. Proteomics. Microbial biotechnology. Industrial microbiology. Recombinant Proteins -- Biosynthesis.
Recombinant protein production has traditionally relied on organisms with well developed molecular biological tools, such as E. coli. However, E. coli is not suited for large industrial scale production due to its propensity to produce organic...
Dartmouth Dissertations

Effects of anoxia on phagocytic function in the pulmonary alveolar macrophage.
Macrophages. ; Pulmonary alveoli. ; Anoxemia.
Dartmouth Dissertations

Evaluation and engineering of Saccharomyces cerevisiae for cellulase expression and growth on insoluble cellulose.
Saccharomyces cerevisiae -- Genetics. Cellulase -- Biotechnology. Cellulose -- Biotechnology. Biomass conversion -- Technological innovations.
Dartmouth Dissertations

Distinct functions for diguanylate cyclases in regulating Pseudomonas aeruginosa PA14 surface-associated behaviors.
Pseudomonas aeruginosa -- Genetics. Biofilms -- Growth. Bacteria -- Motility. Guanylate cyclase. Cellular signal transduction.
Biofilm formation is a complex process culminating in a multicellular microbial structure encased in an extracellular matrix. In order for planktonic cells to initiate and proceed through biofilm formation, they must appropriately regulate a number...
Dartmouth Dissertations

Cellulose hydrolysis and fermentation by Clostridium thermocellum for the production of ethanol.
Clostridium -- Biotechnology. ; Cellulose. ; Hydrolysis. ; Fermentation. ; Alcohol -- Synthesis.
Dartmouth Dissertations

Small molecule modulation of morphology and metabolism in Candida albicans / by Allia Kaye Smith
Candida albicans is a fungal species that naturally exists as a commensal member of the human microbiota, but can assume a pathogenic lifestyle and subsequently induce life-threatening systemic infections. The ability of the fungus to grow in...
Dartmouth Dissertations

Characterization and engineering of alginate lyases: assessing therapeutic revelance
Chronic infection of the human airway by mucoid Pseudomonas aeruginosa is cited as the leading cause of death among Cystic Fibrosis patients. The persistent nature of these infections is linked to P. aeruginosa's ability to form protective biofilm...
Dartmouth Dissertations

The dual role of the Pseudomonas aeruginosa type I-F CRISPR-Cas system in group behavior modification and viral immunity / by Gary E. Heussler
CRISPR-Cas (clustered regularly interspaced short palindromic repeats-CRISPR associated) systems have been characterized as adaptive immune systems in bacteria and archaea, providing defense against mobile genetic elements, including bacteriophage,...
Dartmouth Dissertations

The P. aeruginosa CRISPR/Cas system and its role in bacteria-bacteriophage interactions
CRISPR/Cas systems are a diverse family of small RNA pathways that are commonly described as a resistance mechanism to infection by lytic bacteriophage and invasion by conjugative plasmids. In the current work we demonstrate that CRISPR/Cas systems...
Dartmouth Dissertations

Find results with:

Searching collections:

Add or remove other collections to your search:

Narrow your search by:

Title

Date

Subject

You've searched:

Thumbnail

Title

Subject

Description

Collection

QuickView

Layout options:

		Thumbnail with title
		Grid with smaller thumbnails and more detail

Find results with:

Searching collections:

Add or remove other collections to your search:

Narrow your search by:

Title

Date

Subject

You've searched:

Thumbnail Title Subject Description Collection

QuickView

Layout options:

Thumbnail

Title

Subject

Description

Collection